Chinese Journal OF Rice Science ›› 2017, Vol. 31 ›› Issue (3): 238-246.DOI: 10.16819/j.1001-7216.2017.6156 238
• Orginal Article • Previous Articles Next Articles
Yusong LÜ1,#, Yunfeng XIE1,2,#, Zhonghua SHENG1, Yawen WU1, Shaoqing TANG1, Peisong HU1, Xiangjin WEI1,*()
Received:
2016-11-24
Revised:
2017-02-06
Online:
2017-05-10
Published:
2017-05-10
Contact:
Xiangjin WEI
吕育松1,#, 谢耘丰1,2,#, 圣忠华1, 邬亚文1, 唐绍清1, 胡培松1, 魏祥进1,*()
通讯作者:
魏祥进
基金资助:
CLC Number:
Yusong LÜ, Yunfeng XIE, Zhonghua SHENG, Yawen WU, Shaoqing TANG, Peisong HU, Xiangjin WEI. Morphological and Molecular Genetic Analysis of a Dwarf and Small Grain Rice Variety Xiaoxiang’ai[J]. Chinese Journal OF Rice Science, 2017, 31(3): 238-246.
吕育松, 谢耘丰, 圣忠华, 邬亚文, 唐绍清, 胡培松, 魏祥进. 矮秆小粒水稻潇湘矮的形态学与分子遗传学分析[J]. 中国水稻科学, 2017, 31(3): 238-246.
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URL: http://www.ricesci.cn/EN/10.16819/j.1001-7216.2017.6156 238
引物 Marker name | 前引物序列 Forward(5′-3′) | 后引物序列 Reverse(5′-3′) |
---|---|---|
RM18408 | GGCACTCTGGTTCCTCAATGG | TCTGTATCATCAACGGCAACTCC |
RM5140 | GGCACTCGTATTTCTCAACTTCTCC | GGGTGTATCAGGAGTACAGGTTGC |
RM18457 | ATCCTCCACCGCTCAAGAACACG | CGAGGCCATTCATCGAACAAAGC |
RM18402 | TTATGAGGCAGCCCGTAATGTTGC | GCAGCGGTGTCAACAGCTTCC |
RM18450 | AAGGCTCCATGGTTGGTTGC | CGATGGACAGACAGTGTGTAGTGG |
F78 | GGGACGAATTCTTTTCGATTAC | CGTGGACCAATTTTGGTAACTG |
F79 | AATTATTCCACTATGCACATGT | ATTTTCTTCCATCGCCTCTTGC |
F81 | GTAAACTATCGACTTGCTATGT | ACTAGTGCAGACTGTTTTCCTG |
F82 | AGATGATTGGATGAGAATTTAA | ACCCAGAAACCATCTAGTAATT |
F84 | GGGTGGCTCCTTACGACATTAC | TTTCATATTTTAGCGGTGCTCT |
F85 | CCTCGACCCACTGCATCATCAG | TGGTCGGTCTCCTCCCTCTTCA |
F86 | CAGTATTCGAGTAAGTTCACAA | CCTCGCCCCTATTCATCCTCTT |
Table 1 Primers for mapping in the study.
引物 Marker name | 前引物序列 Forward(5′-3′) | 后引物序列 Reverse(5′-3′) |
---|---|---|
RM18408 | GGCACTCTGGTTCCTCAATGG | TCTGTATCATCAACGGCAACTCC |
RM5140 | GGCACTCGTATTTCTCAACTTCTCC | GGGTGTATCAGGAGTACAGGTTGC |
RM18457 | ATCCTCCACCGCTCAAGAACACG | CGAGGCCATTCATCGAACAAAGC |
RM18402 | TTATGAGGCAGCCCGTAATGTTGC | GCAGCGGTGTCAACAGCTTCC |
RM18450 | AAGGCTCCATGGTTGGTTGC | CGATGGACAGACAGTGTGTAGTGG |
F78 | GGGACGAATTCTTTTCGATTAC | CGTGGACCAATTTTGGTAACTG |
F79 | AATTATTCCACTATGCACATGT | ATTTTCTTCCATCGCCTCTTGC |
F81 | GTAAACTATCGACTTGCTATGT | ACTAGTGCAGACTGTTTTCCTG |
F82 | AGATGATTGGATGAGAATTTAA | ACCCAGAAACCATCTAGTAATT |
F84 | GGGTGGCTCCTTACGACATTAC | TTTCATATTTTAGCGGTGCTCT |
F85 | CCTCGACCCACTGCATCATCAG | TGGTCGGTCTCCTCCCTCTTCA |
F86 | CAGTATTCGAGTAAGTTCACAA | CCTCGCCCCTATTCATCCTCTT |
Fig. 1. Phenotype analysis of XXA and NIL(NIP). A, 5-weeks-old seedlings of NIL(NIP)(left) and XXA(right); B, Phenotypes of NIL(NIP) and XXA plants at the mature stage in paddy field; C, Comparison of main panicles between NIL(NIP) and XXA at the mature stage; D, Grain size of NIL(NIP) and XXA at the mature stage; E-J, Plant height(E), number of panicles per plant(F), seed setting rate(G), 1,000-grain weight(H), grain length(I) and grain width(J) between NIL(NIP) and XXA. Bar=10 cm in Figures A, B and C; Bar=5 mm in Figure D. Data in E-J are shown as Mean±SD from 10 individual replicates. The asterisks indicate statistical significance, as determined by the Student’s t-test(*P<0.05, **P<0.01).
Fig. 2. The internode and panicle length comparison in XXA and NIL(NIP). A, Differences in panicles and internode length for NIL(NIP)(left) and XXA(right) plants. P, Panicle. Those from I to V indicate the corresponding internodes from the top to bottom, bar = 15 cm; B, Internode and young panicle length analysis of NIL(NIP) and XXA at the heading stage. Data in B are shown as Mean±SD from 10 individual replicates. The asterisks indicate statistical significance, as determined by the t-test(**P<0.01).
Fig. 3. Elongation of the second leaf sheath in response to gibberellin(A) and brassinolide(B) in NIL(NIP) and XXA plants. Data in A, B are shown as Mean±SD from 10 individual replicates.
Fig. 4. Fine mapping and positional cloning of the candidate gene. A, Fine mapping of the xxa gene; B, The structure of candidate gene LOC_Os05g26890 and the mutation sites in XXA. Black arrows show 1-bp nonsense mutation in the 5th exon and a 3-bp deletion that resulted in a lysine deletion in the 12th exon of D1.
F1组合 Combination of F1 | 总植株数 Total No. of plants | 正常植株数 No. of half-dwarfing plants | 矮秆小粒植株数 No. of dwarfing plants | 理论比 Expressed ratio | χ2 |
---|---|---|---|---|---|
日本晴/潇湘矮 Nipponbare/XXA | 3223 | 2341 | 782 | 3:1 | 0.078 |
热研1号/潇湘矮 Reyan 1/XXA | 2964 | 2249 | 725 | 3:1 | 0.420 |
0248/潇湘矮 0248/XXA | 3744 | 2812 | 932 | 3:1 | 0.937 |
Y01/潇湘矮 Y01/XXA | 3416 | 2560 | 856 | 3:1 | 0.937 |
Table 2 Segregation of combination Nipponbare/XXA , Reyan 1/XXA, 0248/XXA and Y01/XXA.
F1组合 Combination of F1 | 总植株数 Total No. of plants | 正常植株数 No. of half-dwarfing plants | 矮秆小粒植株数 No. of dwarfing plants | 理论比 Expressed ratio | χ2 |
---|---|---|---|---|---|
日本晴/潇湘矮 Nipponbare/XXA | 3223 | 2341 | 782 | 3:1 | 0.078 |
热研1号/潇湘矮 Reyan 1/XXA | 2964 | 2249 | 725 | 3:1 | 0.420 |
0248/潇湘矮 0248/XXA | 3744 | 2812 | 932 | 3:1 | 0.937 |
Y01/潇湘矮 Y01/XXA | 3416 | 2560 | 856 | 3:1 | 0.937 |
Fig. 5. Phenotype analysis of complementary plants(D1/XXA). A-D, Phenotypes of the complementary plants of D1/XXA-1(right) and XXA(left) at harvesting time, including plant type(A), panicle(B), grain size(C) and internode(D) of D1/XXA-1 and XXA; E, Relative expression levels of D1 gene in XXA and D1/XXA lines. F-I, Internode length(F), plant height(G), grain length(H) and grain width(I) of D1/XXA-1 and XXA(Bar=10 cm in A, B and D; Bar=5 mm in C). Data in E are shown as Mean±SD from 3 individual replicates. Data in F-I are shown as Mean±SD from 10 individual replicates. The asterisks indicate statistical significance, as determined by the Student’s t-test(*P<0.05; **P<0.01).
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